| 不同处理条件对介质阻挡放电低温等离子体杀菌效果及影响机理研究 |
| |
| 引用本文： | 马良军,王佳媚,黄明明,章建浩,严文静.不同处理条件对介质阻挡放电低温等离子体杀菌效果及影响机理研究[J].微生物学报,2019,59(8):1512-1521. |
| |
| 作者姓名： | 马良军 王佳媚 黄明明 章建浩 严文静 |
| |
| 作者单位： | 南京农业大学食品科学技术学院, 江苏省肉类生产与加工质量安全控制协同创新中心, 国家肉品质量安全控制工程技术研究中心, 江苏 南京 210095,南京农业大学食品科学技术学院, 江苏省肉类生产与加工质量安全控制协同创新中心, 国家肉品质量安全控制工程技术研究中心, 江苏 南京 210095;海南大学食品学院, 海南 海口 570228,南京农业大学食品科学技术学院, 江苏省肉类生产与加工质量安全控制协同创新中心, 国家肉品质量安全控制工程技术研究中心, 江苏 南京 210095,南京农业大学食品科学技术学院, 江苏省肉类生产与加工质量安全控制协同创新中心, 国家肉品质量安全控制工程技术研究中心, 江苏 南京 210095,南京农业大学食品科学技术学院, 江苏省肉类生产与加工质量安全控制协同创新中心, 国家肉品质量安全控制工程技术研究中心, 江苏 南京 210095 |
| |
| 基金项目： | 江苏省农业创新资金[CX（18）3041]；南京市农业科技攻关项目（201805038）；国家青年科学基金（31601565） |
| |
| 摘 要： | 【目的】研究不同处理条件下介质阻挡放电低温等离子体对单增李斯特菌和肠炎沙门氏菌的杀菌效果,以及抑菌活性物质含量随处理时间的变化。【方法】以单增李斯特菌和肠炎沙门氏菌为对象,研究不同电压、时间及氧气浓度对介质阻挡放电低温等离子体杀菌效果的影响,通过气态活性物质测定管测定O_3和NO_2浓度随处理时间的变化,利用荧光强度表征菌液中产生的活性物质浓度,采用荧光分光光度法测定·OH、H_2O_2和~1O_2随处理时间的变化。【结果】采用70 kV、150 s或80 kV、1_20 s能够完全杀灭肠炎沙门氏菌,而单增李斯特菌只在80kV、1_20s条件下才能被完全杀死。采用50%O_2+50%N_2的混合气体能够在90 s内完全杀死细菌。随处理时间的增加,两种菌体细胞内活性氧物质含量呈逐渐增加趋势。【结论】电压、时间及氧气浓度均能显著增强等离子体的杀菌效果,其杀菌作用主要与H_2O_2、·OH、1O_2等活性氧物质的含量有关,其中·OH和H_2O_2是介质阻挡放电等离子体杀菌的主要物质。
|
| 关 键 词： | 介质阻挡放电 低温等离子体 活性氧物质 单增李斯特菌 肠炎沙门氏菌 杀菌 |
| 收稿时间： | 2018/10/7 0:00:00 |
| 修稿时间： | 2018/12/12 0:00:00 |
Sterilization by dielectric barrier discharge low temperature plasma under different treatment conditions |
| |
| Liangjun M,Jiamei Wang,Mingming Huang,Jianhao Zhang and Wenjing Yan.Sterilization by dielectric barrier discharge low temperature plasma under different treatment conditions[J].Acta Microbiologica Sinica,2019,59(8):1512-1521. |
| |
| Authors: | Liangjun M Jiamei Wang Mingming Huang Jianhao Zhang and Wenjing Yan |
| |
| Institution: | Jiangsu Collaborative Innovation Center of Meat Production and Processing, National Center of Meat Quality and Safety Control, College of Food Science and Technology, Nanjing Agricultural University, Nanjing 210095, Jiangsu Province, China,Jiangsu Collaborative Innovation Center of Meat Production and Processing, National Center of Meat Quality and Safety Control, College of Food Science and Technology, Nanjing Agricultural University, Nanjing 210095, Jiangsu Province, China;College of Food Science and Technology, Hainan University, Haikou 570228, Hainan Province, China,Jiangsu Collaborative Innovation Center of Meat Production and Processing, National Center of Meat Quality and Safety Control, College of Food Science and Technology, Nanjing Agricultural University, Nanjing 210095, Jiangsu Province, China,Jiangsu Collaborative Innovation Center of Meat Production and Processing, National Center of Meat Quality and Safety Control, College of Food Science and Technology, Nanjing Agricultural University, Nanjing 210095, Jiangsu Province, China and Jiangsu Collaborative Innovation Center of Meat Production and Processing, National Center of Meat Quality and Safety Control, College of Food Science and Technology, Nanjing Agricultural University, Nanjing 210095, Jiangsu Province, China |
| |
| Abstract: | Objective] To study the bactericidal effect of dielectric barrier discharge low temperature plasma on Listeria monocytogenes and Salmonella Enteritidis under different treatment conditions and the content of antibacterial active substances with treatment time. Methods] Listeria monocytogenes and Salmonella enteritidis were used as research objects to determine the total number of colonies after treatment with different voltage, time and oxygen concentration. The concentration of O3 and NO2 was determined by gas-active substance determination tube with treatment time and fluorescence intensity was utilized. The concentration of free radicals generated in the bacterial liquid was characterized and the changes of·OH, H2O2 and 1O2 with the treatment time were determined by fluorescence spectrophotometry. Results] Using 70 kV, 150 s or 80 kV, 120 s could completely kill Salmonella Enteritidis, whereas Listeria monocytogenes could be completely killed only at 80 kV, 120 s. A mixture of 50% O2+50% N2 could completely kill the bacteria in 90 s. With the increase of treatment time, The content of intracellular ROS in two bacteria gradually increased. Conclusion] The voltage, time and oxygen concentration could significantly enhance the bactericidal effect of plasma. Active oxygen radicals such as H2O2 and·OH could promote the bactericidal effect on Listeria monocytogenes and Salmonella enteritidis. Moreover, the bactericidal effect of·OH and H2O2 was better than that of 1O2. |
| |
| Keywords: | dielectric barrier discharge low temperature plasma reactive oxygen species Listeria monocytogenes Salmonella enteritidis sterilization |
| 本文献已被 CNKI 等数据库收录！ |
| 点击此处可从《微生物学报》浏览原始摘要信息 |
| 点击此处可从《微生物学报》下载免费的PDF全文 |
|
