青少年痤疮面部皮肤微生物群落结构变化

【背景】青少年痤疮是一种最常见的慢性炎症性损容性皮肤病,与痤疮丙酸杆菌的异常增殖有关。【目的】探究痤疮皮损区与附近无明显皮损区微生物组成与健康对照的差异,为从微生态角度防治痤疮提供理论基础。【方法】利用细菌16S rRNA基因V1-V2区和真菌TIS1高通量测序技术分析北京地区16岁青少年面部痤疮皮肤细菌和真菌群落结构,将痤疮皮损区与附近无明显皮损区微生物组成与健康组进行比较,寻找差异菌群。【结果】痤疮患者面部皮损区与附近无明显皮损区细菌多样性(Shannon指数)较健康对照组显著性降低(P0.001),主要与丙酸杆菌(痤疮丙酸杆菌)和葡萄球菌(表皮葡萄球菌PM221)显著性上升相关,而痤疮皮损区与附近未明显皮损区细菌组成无显著性差异。痤疮患者皮损区与附近无明显皮损区较健康对照组真菌丰富度(Chao1指数)显著性上升(P0.05),与限制性马拉色菌的显著上升相关。【结论】面部皮肤微生物变化与青少年痤疮的发生相关。本研究为从微生物角度防治痤疮提供理论依据。

Variation of skin microbial community in adolescent acne

[Background] Adolescents acne is one of the most common chronic inflammatory dermatitis associated with abnormal proliferation of Propionibacterium acnes. [Objective] The aim of this study was to explore the difference of microbial composition between the acne skin and healthy control, and to provide theoretical basis for the prevention and treatment of acne from the perspective of microecology. [Methods] Bacterial 16S rRNA gene V1?V2 region sequencing and fungal TIS1 region sequencing technology were used to analyze the bacterial and fungal community structure of facial acne skin in 16-year-old teenagers in Beijing. Bacterial and fungal composition in adolescent acne with lesions and nearby areas without obvious lesions, as well as healthy controls, were investigated. [Results] Compared to the healthy samples, the skin bacterial diversity of adolescent with acne was significantly decreased (P<0.001), and the abundance of Propionibacterium (P. acnes) and Staphylococcus (Staphylococcus epidermidis PM221) decreased significantly. While there was no significant difference in the bacterial composition between the acne lesion area and the nearby no obvious lesion area. The fungal richness (Chao1 index) and the abundance of Malassezia restricta of the adolescent acne group was significantly higher than that of the healthy control group (P<0.05). [Conclusion] The change of skin microbial composition is associated with the occurrence of adolescents acne. This study provided theoretical basis for the prevention and treatment of acne from the perspective of microorganisms.