Minimal DNA requirement for topoisomerase II-mediated cleavage in vitro |
| |
| Authors: | K Lund A H Andersen K Christiansen J Q Svejstrup O Westergaard |
| |
| Affiliation: | Department of Molecular Biology and Plant Physiology, University of Aarhus, Denmark. |
| |
| Abstract: | The minimal DNA requirement for topoisomerase II-mediated DNA cleavage in vitro was determined by analyzing the interaction of the enzyme with sets of DNA substrates varying successively by single bases at the 5'- or 3'-end of either strand. A 16-base pair double-stranded region was established as the minimal duplex region required for topoisomerase II cleavage activity. The region was located symmetrically around the 4-base staggered cleavage site. Topoisomerase II-mediated cleavage within the 16-base pair core duplex, however, required single-stranded regions flanking the duplex to either the 5'- or 3'-sides, or an extension at both ends of the duplex with 1 or more base pairs. |
| |
| Keywords: | |
|
|
