Design of an expression vector and its application to heterologous protein expression in Bacillus subtilis |
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Authors: | Min-Young So Cheol-Ho Pan Sangkee Rhee Su-Il Kim |
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Institution: | (1) School of Agricultural Biotechnology, Seoul National University, Suwon, 441-744, Korea |
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Abstract: | An expression vector, pUBEX, was constructed for extracellular production of heterologous proteins in Bacillus subtilis using a polyhistidine tag on the C-terminal sequence, providing an efficient and easy purification of the protein. A CII protein, a member of Bowman–Birk protease inhibitors, which was expressed as an inactive protein in Escherichia coli, was successfully expressed in Bacillus subtilis using the pUBEX vector and was purified to 6.4 mg l–1 by the immobilized metal affinity chromatography. |
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Keywords: | Bacillus subtilis Bowman– Birk protease inhibitor inclusion body polyhistidine tag protein expression |
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