Construction of a Multihybrid Display System: Flagellar Filaments Carrying Two Foreign Adhesive Peptides |
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| Authors: | Jarna Tanskanen Timo K Korhonen and Benita Westerlund-Wikstrm |
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| Institution: | Jarna Tanskanen, Timo K. Korhonen, and Benita Westerlund-Wikström |
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| Abstract: | A multivalent, bifunctional flagellum carrying two different adhesive peptides in separate flagellin subunits within a filament was constructed in Escherichia coli. The inserted peptides were the fibronectin-binding 115-mer D repeat region of Staphylococcus aureus and the 302-mer collagen-binding region of YadA of Yersinia enterocolitica. Western blotting, immunoelectron microscopy, and adhesion tests with hybrid flagella from an in trans-complemented ΔfliC E. coli strain showed that individual filaments consisted of both recombinant flagellins. |
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