Investigation of the physiological properties and synthesis of PUFAs from Thraustochytrids and its electrophoretic karyotypes |
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Authors: | Periasamy Anbu Dong-Uk Kim Eun-Jin Jeh Young-Su Jeong Byung-Ki Hur |
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Institution: | (1) Department of Biological Engineering, Inha University, 402-751 Incheon, Korea;(2) Department of Biological Sciences, Korea Advanced Institute of Science and Technology, 305-701 Daejeon, Korea |
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Abstract: | Physiological properties of organism, such as the number of chromosomes, genome size, fatty acid profile and the activities
of desaturases and elongases were investigated for four differentThraustochytrium species. The investigation revealed thatThraustochytrium aureum could synthesize a significant level of polyunsaturated fatty acids (PUFAs), particularly docosa-hexaenoic acid (DHA), when
compared to the other threeThraustochytrium species tested. A higher level of saturated fatty acids was observed byT. striatum followed byThraustochytrium sp. ATCC 26185. The PUFA accumulation rate was higher in the n-3 than in the n-6 pathway. A comparison of the activities
for these desaturases and elongases of the four different species were also studied. Further, the electrophoretic karyotypes
of Thraustochytrids were separated by pulsed-field gel electrophoresis (PFGE). The separation condition of PFGE was developed
to obtain the different chromosomes from the variousThraustochytrium species. The number of chromosomes inT. aureum, T. striatum, Thraustochytrium sp. ATCC 20891 andThraustochytrium sp. ATCC 26185 were 13, 17, 10. 8, and the whole genome size of those species were estimated to be 12.9, 11.7, 11.3 and 9.93
Mbp, respectively. |
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Keywords: | chromosome polyunsaturated fatty acids fatty acid profiles pulsed-field gel electrophoresis Thraustochytriumspecies |
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