Single-tube detection of nine bacterial antibiotic-resistance genes by a 2-dimensional multiplex qPCR assay based on fluorescence and melting temperature |
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Authors: | Li Yingxue Xu Ping Wan Zhenzhou Du Hong Jin Xia Zhang Chiyu |
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Affiliation: | 1.Shanghai Public Health Clinical Center, Fudan University, Shanghai, 201508, China ;2.Pathogen Discovery and Evolution Unit, Institut Pasteur of Shanghai, Chinese Academy of Science, Shanghai, 200031, China ;3.School of Life Sciences, Shanghai University, Shanghai, 200444, China ;4.Department of Clinical Laboratory, The Fifth People’s Hospital of Suzhou, Suzhou, 215000, China ;5.Medical Laboratory of Taizhou Fourth People’s Hospital, Taizhou, 225300, Jiangsu, China ;6.Department of Clinical Laboratory, The Second Affiliated Hospital of Soochow University, Suzhou, 215000, China ; |
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Abstract: | Molecular Biology Reports - Simple, multiplex qPCR methods are advantages for rapid molecular diagnosis of multiple antibiotics-resistant genes simultaneously. However, the number of genes can be... |
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