Inline index helped in cleaning up data contamination generated during library preparation and the subsequent steps期刊界 All Journals 搜尽天下杂志传播学术成果专业期刊搜索期刊信息化学术搜索

按检索

Inline index helped in cleaning up data contamination generated during library preparation and the subsequent steps

Authors:	Wang Ying Yuan Hao Huang Junman Li Chenhong

Institution:	1.Shanghai Universities Key Laboratory of Marine Animal Taxonomy and Evolution, Shanghai Ocean University, Shanghai, 201306, China ;2.Shanghai Collaborative Innovation for Aquatic Animal Genetics and Breeding, Shanghai Ocean University, Shanghai, 201306, China ;

Abstract:	Background High-throughput sequencing involves library preparation and amplification steps, which may induce contamination across samples or between samples and the environment. Methods We tested the effect of applying an inline-index strategy, in which DNA indices of 6 bp were added to both ends of the inserts at the ligation step of library prep for resolving the data contamination problem. Results Our results showed that the contamination ranged from 0.29 to 1.25% in one experiment and from 0.83 to 27.01% in the other. We also found that contamination could be environmental or from reagents besides cross-contamination between samples. Conclusions Inline-index method is a useful experimental design to clean up the data and address the contamination problem which has been plaguing high-throughput sequencing data in many applications.

Keywords:
本文献已被 SpringerLink 等数据库收录！