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Rapid visual genotyping method for germline mutants with small genomic fragment deletion by allele-specific PCR and lateral flow nucleic acid biosensor |
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| Authors: | Su Qiuju Zhou Xiang Wu Tianwen Li Kui Xu Wentao Lin Zhenyu Shen Ping Liu Bang |
| Institution: | 1.Key Laboratory of Agricultural Animal Genetics, Breeding, and Reproduction of Ministry of Education, Huazhong Agricultural University, Wuhan, 430070, China ;2.The Cooperative Innovation Center for Sustainable Pig Production, Wuhan, 430070, China ;3.Institute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing, 100193, China ;4.Beijing Advanced Innovation Center for Food Nutrition and Human Health, College of Food Science & Nutritional Engineering, China Agricultural University, 100083, Beijing, China ;5.MOE Key Laboratory of Analysis and Detection for Food Safety, Fujian Provincial Key Laboratory of Analysis and Detection Technology for Food Safety, Department of Chemistry, Fuzhou University, Fuzhou, 350002, Fujian, China ;6.Development Center for Science and Technology, Ministry of Agriculture and Rural Affairs, Beijing, 100045, China ; |
| Abstract: | Molecular Biology Reports - Genome-editing techniques incorporating artificial nucleases develop rapidly and enable efficient and precise modification of genomic DNA of numerous organisms. The... |
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