Tendamistat surface accessibility to the TEMPOL paramagnetic probe |
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Authors: | Maria Scarselli Andrea Bernini Claudia Segoni Henriette Molinari Gennaro Esposito Arthur M Lesk Franco Laschi Pierandrea Temussi Neri Niccolai |
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Institution: | (1) Dipartimento di Biologia Molecolare and Centro per lo Studio Strutturale di Sistemi Biomolecolari, Università di Siena, Via A. Fiorentina 1, I-53100 Siena, Italy;(2) Istituto Policattedra, Università di Verona, I-37100 Verona, Italy;(3) Dipartimento di Scienze e Tecnologie Biomediche, Università di Udine, I-33100 Udine, Italy;(4) Department of Haematology, University of Cambridge, Cambridge, CB2 2QH, U.K.;(5) Dipartimento di Chimica, Università di Siena, I-53100 Siena, Italy;(6) Dipartimento di Chimica, Università di Napoli, I-80134 Napoli, Italy |
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Abstract: | TEMPOL, the soluble spin-label 4-hydroxy-2,2,6,6-tetramethyl-piperidine-1-oxyl, has been used to determine the surface characteristics of tendamistat, a small protein with a well-characterised structure both in solution and in the crystal. A good correlation has been found between predicted regions of exposed protein surface and the intensity attenuations induced by the probe on 2D NMR TOCSY cross peaks of tendamistat in the paramagnetic water solution. All the high paramagnetic effects have been interpreted in terms of more efficient competition of TEMPOL with water molecules at some surface positions. The active site of tendamistat coincides with the largest surface patch accessible to the probe. A strong hydration of protein N and C termini can also be suggested by this structural approach, as these locations exhibit reduced paramagnetic perturbations. Provided that the solution structure is known, the use of this paramagnetic probe seems to be well suited to delineate the dynamic behaviour of the protein surface and, more generally, to gain relevant information about the molecular presentation processes. |
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Keywords: | molecular presentation protein structure spin-labels surface accessibility tendamistat |
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