| 基于硫醇自组装的肌红蛋白单克隆抗体金膜固相载体的构建 |
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| 引用本文： | 雷博,曲炳郡,杨华,张向颖,刘芳.基于硫醇自组装的肌红蛋白单克隆抗体金膜固相载体的构建[J].生物工程学报,2011,27(10):1527-1535. |
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| 作者姓名： | 雷博 曲炳郡 杨华 张向颖 刘芳 |
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| 作者单位： | 1. 清华大学微电子学研究所,北京,100084
2. 北京市肝病研究所,北京,100069 |
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| 基金项目： | 国家高技术研究发展计划 (863 计划) (No. 2006AA03Z317) 资助。 |
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| 摘 要： | 为了在金膜固相载体上固定肌红蛋白单克隆抗体 (MbAb)，通过在金膜上生长一层巯基酸和巯基醇的混合自组装分子膜 (SAMs)，用原子力显微镜 (AFM) 和X射线光电子能谱仪 (XPS) 分析样品的性质，再以1-(3-二甲基氨基丙基) 3-乙基碳化二亚胺盐酸 (EDC·HCl) 作为催化剂，自组装膜和抗体的氨基发生偶联反应，将抗体固定在金膜表面，并进行肌红蛋白抗原 (MbAg) 的测定。结果显示，通过条件优化实验，发现50 mmol/L的巯基16酸和巯基11醇混合乙醇溶液，60 ℃处理金膜3 h后，再偶联
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| 关 键 词： | 肌红蛋白，心肌梗塞，硫醇自组装膜，金膜固相载体，单克隆抗体，磁电阻生物传感器 |
| 收稿时间： | 3/3/2011 12:00:00 AM |
Construction of gold film solid-phase carrier of myoglobin monoclonal antibody based on thiol self-assembly |
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| Bo Lei,Bingjun Qu,Hua Yang,Xiangying Zhang and Fang Liu.Construction of gold film solid-phase carrier of myoglobin monoclonal antibody based on thiol self-assembly[J].Chinese Journal of Biotechnology,2011,27(10):1527-1535. |
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| Authors: | Bo Lei Bingjun Qu Hua Yang Xiangying Zhang and Fang Liu |
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| Institution: | Institute of Microelectronics, Tsinghua University, Beijing 100084, China;Institute of Microelectronics, Tsinghua University, Beijing 100084, China;Institute of Microelectronics, Tsinghua University, Beijing 100084, China;Beijing Institute of Liver Diseases, Beijing 100069, China;Beijing Institute of Liver Diseases, Beijing 100069, China |
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| Abstract: | In order to immobilize myoglobin(Mb) monoclonal antibody on gold film solid-phase carrier,we grew a mixed self-assembled monolayers(SAMs) of acid thiol and mercapto ethanol on gold film.Then we analyzed the property of the sample by atomic force microscopy and X-ray photoelectron spectroscopy.Then,we used 1-(3-dimethyl aminopropyl)-3-ethyl carbodiimide hydrochloride as catalyst to couple SAMs with amino of antibody so that we immobilized antibody on surface of gold film,followed by detecting myoglobin antig... |
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| Keywords: | myoglobin myocardial infarction thiol self-assembled monolayers gold film solid-phase carrier monoclonal antibody magnetoresistence biosensors |
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