Plant tissue culture. Biotechnology. Milestones |
| |
Authors: | Email author" target="_blank">Oluf?L?GamborgEmail author |
| |
Institution: | (1) 1404 Solana Drive, 94002 Belmont, CA |
| |
Abstract: | Summary The progress in the development of the technologies of plant tissue and cell culture over the past four decades has been remarkable.
This article covers my personal reflections on the various topics and is based on my involvement in the field during that
period. There are three fundamental technologies which constitute most of what is referred to as plant in vitro technologies or tissue culture. The origin and some of the key persons involved in the development of each of these procedures
will be discussed. The technology that is most common is growing plant tissue on gel-solidified nutrient media. That technology
is being used in the most vital procedures, namely the regeneration of plants from cultured cells. The culture of plant cells
in liquid suspension was developed very shortly after that, and has become a very effective technology for plant regeneration
by somatic embryogenesis. The method of meristem culture arose out of a need for developing plants that were virus-free. In
many species the technique is now being used to produce virus-free crop plants. Another important technology is the culture
of anthers and microspores for producing haploid and homozygous plants. Included with plant tissue culture is the development
of the plant protoplast and cell fusion technologies for the production of new plant hybrids. The final aspect of the development
concerns the integration of tissue culture with molecular genetics, which has developed into the rapidly expanding field of
biotechnology. |
| |
Keywords: | anther/microspore culture cell suspension culture gene transfer genome sequencing in vitro technologies meristem culture phytochemicals plant regeneration protoplasts |
本文献已被 SpringerLink 等数据库收录! |