| 应用环磷酰胺研制细胞表面抗原的单克隆抗体 |
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| 引用本文： | 周王景,马宝骊,严俊. 应用环磷酰胺研制细胞表面抗原的单克隆抗体[J]. 微生物学免疫学进展, 2000, 0(3) |
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| 作者姓名： | 周王景 马宝骊 严俊 |
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| 作者单位： | 上海第二医科大学免疫学教研室!上海200025 |
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| 摘 要： | 利用环磷酰胺和杂交瘤技术 ,成功制备了 1株稳定分泌抗人CD80 分子单克隆抗体的杂交瘤细胞。实验结果表明 ,该单抗腹水效价为 1 0 6 ,属IgG1亚类 ,特异性强 ,为今后的应用打下了基础。
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| 关 键 词： | 环磷酰胺 免疫耐受 hCD80 McAb |
The preparation of monoclonal antibody against cell membrane by cyclophosphamide |
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| ZHOU Jing,MA Bao li,YAN Jun. The preparation of monoclonal antibody against cell membrane by cyclophosphamide[J]. Progress In Microbiology and Immunology, 2000, 0(3) |
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| Authors: | ZHOU Jing MA Bao li YAN Jun |
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| Abstract: | One stable hybridoma cell line secreting McAb anti CD 80 has been established.The McAb belongs to IgG1 subclass.The titer of McAb in ascites was 10 6 .The specificity of McAb were identified by cell ELISA and FACS.The results above have laid foundation for the application. |
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| Keywords: | Cyclophosphamide Immune tolerance hCD 80 McAb |
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