| 幽门螺杆菌尿素酶的纯化及其特性 |
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| 引用本文： | 陈爽,李立.幽门螺杆菌尿素酶的纯化及其特性[J].微生物学免疫学进展,2000,28(4):37-42. |
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| 作者姓名： | 陈爽 李立 |
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| 作者单位： | 长春生物制品研究所,长春 |
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| 摘 要： | 本文利用Ｓｅｐｈａｃｒｙ１ Ｓ－２００和Ｑ－Ｓｅｐｈａｒｏｓｅ两步层析，从ＨＰ蒸馏水浸液中提取纯化尿素酶，并对其特性进行了测定，证明ＨＰ尿素酶各含一个６６ｋＤ和２９．５ｋＤ的亚单位，并以６个分子聚合成６２５ｋＤ大分子蛋白，有很好的抗原性，并显示特异的血清学反应。用ＨＰ超声浸液抗原和尿素酶，加入２μｇ霍乱毒素粘膜佐剂，给ＳＰＦ ＢＡＬＢ／Ｃ小鼠口服免疫，可使８０％小鼠抵抗ＨＰ活菌的攻击，证明尿素酶是一种抗ＨＰ的保护性抗原。
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| 关 键 词： | 尿素酶 柱层析 SDS－PAGE 纯化 特性 幽门螺杆菌 |
Purification urease from Helicobacter Pylori and analysis is properties |
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| CHEN Shuang,LI Li,SHEN Wen sheng,et al..Purification urease from Helicobacter Pylori and analysis is properties[J].Progress In Microbiology and Immunology,2000,28(4):37-42. |
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| Authors: | CHEN Shuang LI Li SHEN Wen sheng |
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| Abstract: | H.pylori urease was purified with sephacry1 S 200 and Q Sepharose colum from H.pylori that had subjected to ultrasonication.The preparation only contained H.pylori urease(SDS PAGE showed 66 kD and 29.5 kD bands only).It has an excellent antigeneicity and gives rise to specific antibody response. SPF BALB/C mice were immunized by purified urease, sonicated HP plus cholera toxia(CT), The protection rate was 80%. The results showed that urease was an anti HP protective antigen. |
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| Keywords: | H pylori Urease Column chromalography SDS PAGE Western blot Ion exchange chromatography |
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