| 分批培养时pH和温度对重组大肠杆菌生产谷胱甘肽合成酶系的影响 |
| |
| 引用本文： | 童群义,陈坚. 分批培养时pH和温度对重组大肠杆菌生产谷胱甘肽合成酶系的影响[J]. 工业微生物, 2001, 31(4): 17-21 |
| |
| 作者姓名： | 童群义 陈坚 |
| |
| 作者单位： | 江南大学生物工程学院,无锡214036 |
| |
| 基金项目： | 霍英东高等院校青年教师基金，江苏省跨世纪学科带头人科研基金资助 |
| |
| 摘 要： | 研究了2．5L罐分批培养时pH和温度对重组大肠杆菌生产谷胱甘肽合成酶系的影响，确定了分批培养时生产谷胱甘肽合成酶系的最佳pH和最佳温度。研究结果表明：在发酵液的pH为7．2和温度为37℃时，谷胱甘肽合成酶系产量和细胞干重达到最大，分别为690．6U／L和3．77g／L。采用变温控制对菌体的生长和谷胱甘肽合成酶系的合成并没有明显的优点。
|
| 关 键 词： | pH 温度控制 分批培养 谷胱甘肽合成酶系 重组大肠杆菌 |
Effects of pH and temperature on the production of glutathione synthetases by recombinant E. coli in batch culture |
| |
| TONG Qun-yi,CHEN Jian. Effects of pH and temperature on the production of glutathione synthetases by recombinant E. coli in batch culture[J]. Industrial Microbiology, 2001, 31(4): 17-21 |
| |
| Authors: | TONG Qun-yi CHEN Jian |
| |
| Abstract: | Effects of pH and temperature on the production of glutathione synthetases by recombinant E.coli were investigated, and the optimal pH and optimal temperature was obtained. The results showed that the maximum yield of glutathione synthetases and the maximum biomass was 690.6U/L and 3.77g/L, respectively. The control strategy of temperature-shift did not have the obvious advantage in the growth and the production of glutathione synthetases. |
| |
| Keywords: | pH temperature batch culture glutathione synthetases |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
