Targeted mapping of ESTs linked to the adult plant resistance gene Lr46 in wheat using synteny with rice |
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Authors: | Maria Mateos-Hernandez Ravi P Singh Scot H Hulbert Robert L Bowden Julio Huerta-Espino Bikram S Gill Gina Brown-Guedira |
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Institution: | (1) Department of Plant Pathology, Kansas State University, Manhattan, KS 66506, USA;(2) Wheat program, CIMMYT, Apartado Postal 6-641, 06600 Mexico, DF, Mexico;(3) Plant Science and Entomology Research Unit, USDA-ARS, 4008 Throckmorton Hall, Kansas State University, Manhattan, KS 66506, USA;(4) Campo Experimental Valle de Mexico, INIFAP, Apdo. Postal 10, Chapingo, 56230, Edo de Mexico, Mexico;(5) USDA-ARS, North Carolina State University, 840 Main Campus Drive, Raleigh, NC 27606, USA |
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Abstract: | The gene Lr46 has provided slow-rusting resistance to leaf rust caused by Puccinia triticina in wheat (Triticum aestivum), which has remained durable for almost 30 years. Using linked markers and wheat deletion stocks, we located Lr46 in the deletion bin 1BL (0.84–0.89) comprising 5% of the 1BL arm. The distal part of chromosome 1BL of wheat is syntenic
to chromosome 5L of rice. Wheat expressed sequence tags (ESTs) mapping in the terminal 15% of chromosome 1BL with significant
homology to sequences from the terminal region of chromosome 5L of rice were chosen for sequence-tagged site (STS) primer
design and were mapped physically and genetically. In addition, sequences from two rice bacterial artificial chromosome clones
covering the targeted syntenic region were used to identify additional linked wheat ESTs. Fourteen new markers potentially
linked to Lr46 were developed; eight were mapped in a segregating population. Markers flanking (2.2 cM proximal and 2.2 cM distal) and cosegregating
with Lr46 were identified. The physical location of Lr46 was narrowed to a submicroscopic region between the breakpoints of deletion lines 1BL-13 fraction length (FL)=0.89–1] and
1BL-10 (FL=0.89–3). We are now developing a high-resolution mapping population for the positional cloning of Lr46. |
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Keywords: | Synteny Rice Wheat Leaf-rust resistance |
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