The activity of exogenous genetic constructs introduced into cells by the technique of ballistic transfection in mouse ontogenesis |
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| Authors: | R A Shafei I A Zelenina M L Semenova V A Kolesnikov V M Mikhailov V S Baranov A V Zelenin V A Golichenkov |
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| Institution: | (1) Moscow State University, 119899 Moscow, Russia;(2) Engel’hardt Institute of Molecular Biology, Russian Academy of Sciences, 117984 Moscow, Russia;(3) Institute of Cytology, Russian Academy of Sciences, 194064 St. Petersburg, Russia;(4) Ott Institute of Obstetrics and Gynecology, Russian Academy of Medical Sciences, 199034 St. Petersburg, Russia |
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| Abstract: | We used the method of particle bombardment (ballistic transfection) to introduce β-galactosidase and human dystrophin genes
into mouse embryos and skeletal muscles of adult mice. We examined the mechanisms of DNA transfer into skeletal muscle cells,
the biological processes accompanying and following this transfer, the susceptibility of various types of muscle cells to
transfection, and the duration of expression of and conditions affecting the introduced genes. We have also developed an effective,
convenient, and practical methods of skeletal muscle transfection. |
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| Keywords: | ballistic transfection (particle bombardment method) transgenic animals gene therapy |
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