Purification and characterization of mannose/glucose-specific lectin from seeds of <Emphasis Type="Italic">Trigonella foenumgraecum</Emphasis> |
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Authors: | Aabgeena Naeem Ejaz Ahmad Mohd Tashfeen Ashraf Rizwan Hasan Khan |
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Institution: | (1) Interdisciplinary Biotechnology Unit, Aligarh Muslim University, Aligarh, 202002, India |
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Abstract: | A lectin present in seeds of Trigonella foenumgraecum was isolated and purified by acid precipitation, salt fractionation, and affinity chromatography on mannan cross-linked agarose.
SDS-PAGE revealed a single band corresponding to a molecular weight of 27,350 daltons. The lectin agglutinated trypsin-treated
rat erythrocytes. Sugar specificity as determined by hemagglutination inhibition assay indicated that the lectin belongs to
a glucose/mannose-specific group. The reaction of the lectin with glycoprotein was affected by pH changes. The carbohydrate
binding specificity of the lectin was investigated by turbidity and activity measurements. As the lectin belongs to the Leguminoceae
family, the specificity of the lectin for glucose/mannose renders it a valuable tool for Rhizobium-legume symbiosis.
Published in Russian in Biokhimiya, 2007, Vol. 72, No. 1, pp. 52–57. |
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Keywords: | Trigonella foenumgraecum legume lectin affinity chromatography carbohydrate binding glucose/mannose-specific |
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