Production and purification of an extracellularly produced K4 polysaccharide from Escherichia coli |
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| Authors: | M Manzoni S Bergomi F Molinari V Cavazzoni |
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| Institution: | (1) Dipartimento di scienze e tecnologie alimentari e microbiologiche, Sezione di microbiologia industriale, Universitá Degli Studi di Milano via Celoria, 2 - 20133 Milano, Italia |
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| Abstract: | Summary The production of the K4 polysaccharide was obtained for the first time extracellularly from a strain of Escherichia coli. The set up of the fermentation conditions led to the maximum fermentation yield, as extracellular K4, after 20 h. Purification and characterization of this K4 resulted in 200 mg/L of highly purified K4. |
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