Labeling of Bifidobacterium longum cells with 13C-substituted leucine for quantitative proteomic analyses |
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| Authors: | Couté Yohann Hernandez Céline Appel Ron D Sanchez Jean-Charles Margolles Abelardo |
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| Affiliation: | Biomedical Proteomics Research Group, Department of Structural Biology and Bioinformatics, University of Geneva, 1 Rue Michel Servet, 1211 Geneva 14, Switzerland. |
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| Abstract: | Stable isotope labeling of amino acids in cell culture was used for Bifidobacterium longum. A comprehensive proteomic strategy was developed and validated by designing an appropriate semidefined medium that allows stable replacement of natural leucine by [(13)C6]leucine. Using this strategy, proteins having variations of at least 50% in their expression rates can be quantified with great confidence. |
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