| 草莓叶片再生芽及遗传转化系统的建立 |
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| 引用本文： | 邓馨 胡文玉. 草莓叶片再生芽及遗传转化系统的建立[J]. 植物学报, 2000, 17(2): 174-178 |
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| 作者姓名： | 邓馨 胡文玉 |
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| 作者单位： | 1(中国科学院植物研究所 北京 100093) 2(沈阳农业大学 沈阳 110161) |
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| 摘 要： | 本文从五个草莓品种中筛选了再生频率高的品种M14,并进一步提高其再生频率至100%,单叶盘再生芽数至10.0个;石蜡切片法观察其芽多起源于愈伤组织,少数由叶细胞脱分化后直接形成; 该再生体系用于根癌农杆菌菌株EHA105介导的基因转化，并以14.5%频率得到抗卡那霉素的抗性芽,初步鉴定是转化芽。上述结果表明高频率稳定的草莓品种M14 叶片再生芽及遗传转化系统已经形成，并可以用于转基因研究。
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| 关 键 词： | 草莓(Fragaria×ananassa Duch.) 再生芽 转基因 |
| 收稿时间： | 1998-10-30 |
| 修稿时间： | 1999-04-03 |
Establishment of Regeneration and Genetic Transformation System of Strawberry Leaf |
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| DENG Xin HU Wen-Yu. Establishment of Regeneration and Genetic Transformation System of Strawberry Leaf[J]. Bulletin of Botany, 2000, 17(2): 174-178 |
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| Authors: | DENG Xin HU Wen-Yu |
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| Affiliation: | 1(Institute of Botany, The Chinese Academy of Sciences, Beijing 100093) 2(Shenyang Agricultural University, Shenyang 110161) |
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| Abstract: | An ideal regeneration system from leaf of strawberry cultivar M14 was developed. 10.0 shoots per leaf disc were generated at the rate of 100% within 3 weeks. Histological observations indicated that the shoots regenerated mainly from calli. This regeneration system was used in genetic transformation mediated by Agrobacterium tumefaciens strain (EHA105) carrying the modified pMDG410 plasmid, which contains GUS and NPTⅡgene. Kanamycin_resistant shoots regenerated with the ratio of 14.5% were identified as transgenic shoots. |
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| Keywords: | ; |
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