DNA sequences of tobacco chloroplast genes for tRNASer (GGA), tRNAThr (UGU), tRNALeu (UAA), tRNAPhe (GAA): the tRNALeu gene contains a 503 bp intron |
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| Authors: | Kyoji Yamada Kazuo Shinozaki Masahiro Sugiura |
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| Institution: | (1) Center for Gene Research, Nagoya University, Chikusa, 464 Nagoya, Japan;(2) Present address: Bioscience Research Laboratories, Yamanouchi Pharmaceutical Co., LTD., Azusawa 1-1-8, Itabashi-ku, 174 Tokyo |
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| Abstract: | Summary The location and nucleotide sequence of tobacco chloroplast genes for tRNASer (GGA), tRNAThr (UGU), tRNALeu (UAA) and tRNAPhe (GAA) (trnS-GGA, trnT-UGU, trnL-UAA and trnF-GAA, respectively) have been determined. These genes are located in the 10 kbp BamHI fragment which lies in the middle of the large single-copy region of the chloroplast DNA. The gene order is trnS-trnT-trnL-trnF. The trnS, trnL and trnF are encoded on the same strand while the trnT on the opposite strand. The trnL contains a 503 bp intron like maize and broad bean trnL-UAAs. |
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| Keywords: | chloroplast cloning DNA sequence intron tobacco tRNA genes |
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