草鱼、中华鳖IL-2活性影响因素的研究

结果显示:当年草鱼种和中华鳖脾细胞培养上清液中能检测出IL-2活性,而且1龄以上草鱼、中华鳖的IL-2活性高于当年孵化的草鱼和中华鳖.草鱼、中华鳖脾细胞在25℃培养温度条件下,其上清液中IL-2活性最高,35℃次之,15℃最低.因此,草鱼、中华鳖IL-2活性在一定范围内是随着年龄增加而增强和依赖温度的.通过小鼠胸腺细胞增殖和对小鼠L929细胞杀伤率的实验表明37℃比25℃检测温度下所测的IL-2活性高,而中华鳖胸腺细胞增殖实验却显示25℃检测温度下IL-2活性高于37℃.本文讨论了年龄、温度对免疫反应影响的机理.  

A STUDY OF INFLUENTIAL FACTORS ON IL-2 ACTIVITY OF GRASSCARP,CTENOPHARYNGODON IDELLUS AND CHINESESOFT-SHELLED TURTLE,TRIONYX SINENSIS

Interleukin 2(IL-2)activities were tested in splenocyte culture supernatant of grass carp, Ctenopharyngodon idellus and Chinese soft-shelled turtle, Trionyx sinensis under one age. IL-2 activities of grass carp and Chinese soft-shelled turtle above one age were higher than those under one age. Within 15℃ to 35℃ range,IL-2 activities of splenocyte culture supernatant of grass carp and Chinese soft-shelled turtle were the highest at 25℃,the lowest at 15℃. Thereby,IL-2 activities increased with age and depended on temperature within a certain range. However,effects of assay temperature on IL-2 activity of grass carp and Chinese soft-shelled turtle were complicated. By thymocyte(C57BL/6-8 mice)proliferation and L929 cytotoxicity assay,IL-2 activities of grass carp and Chinese soft-shelled turtle at 37℃ was higher than those at 25℃. But by thymocyte(T.sinensis)proliferation assay,IL-2 activity of Chinese soft-shelled turtle at 25℃ was higher than those at 37℃. The influential mechanism of age and temperature on immune response has been discussed.