Maleylacetate reductase of Pseudomonas sp. strain B13: dechlorination of chloromaleylacetates,metabolites in the degradation of chloroaromatic compounds |
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Authors: | Stefan R. Kaschabek Walter Reineke |
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Affiliation: | (1) Chemische Mikrobiologie der Bergischen Universität — Gesamthochschule Wuppertal, Gaussstrasse 20, W-5600 Wuppertal 1, Federal Republic of Germany |
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Abstract: | The maleylacetate reductase of 3-chlorobenzoate-grown cells of Pseudomonas sp. strain B13 has been purified 50-fold. The enzyme converted 2-chloromaleylacetate to 3-oxoadipate with temporary occurrence of maleylacetate; 1 mol of chloride was eliminated during the conversion of 1 mol of 2-chloro- and 2,3-dichloromaleylacetate; 2 mol of NADH were consumed per mol of 2-chloro- and 2,3-dichloromaleylacetate while only 1 mol was necessary to catalyze the conversion of maleylacetate or 2-methylmaleylacetate. The maleylacetate reductase failed to use fumarylacetate as a substrate. The role of the enzyme in the chloroaromatics degradation is discussed. |
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Keywords: | 2-Chloromaleylacetate 2,3-dichloromaleylacetate Pseudomonas sp. strain B13 Maleylacetate reductase |
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