| 干扰视黄醇结合蛋白4对猪脂肪细胞PI3K/Akt信号通路的影响 |
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| 引用本文： | 蒲蕾,程佳,吴国芳,杨浩,仇杨,张振宇,杨公社,孙世铎. 干扰视黄醇结合蛋白4对猪脂肪细胞PI3K/Akt信号通路的影响[J]. 生物工程学报, 2013, 29(4): 447-457 |
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| 作者姓名： | 蒲蕾 程佳 吴国芳 杨浩 仇杨 张振宇 杨公社 孙世铎 |
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| 作者单位： | 西北农林科技大学动物脂肪沉积与肌肉发育实验室,陕西杨凌,712100 |
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| 基金项目： | 国家转基因生物新品种培育重大专项 (No. 2011ZX08006-005)，科技部技术推广项目 (No. K332021202)，西北农林科技大学创新团队计划资助。 |
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| 摘 要： | 视黄醇结合蛋白4(Retinol binding protein 4,RBP4)是一种脂肪细胞分泌因子,其表达水平的升高与胰岛素抵抗及Ⅱ型糖尿病等疾病密切相关,但具体作用机制尚不清楚。为明确此机制,通过包装RBP4干扰慢病毒并侵染猪前体脂肪细胞。运用胰岛素激活及诱导胰岛素抵抗模型,利用QRT-PCR及Western blotting方法检测RBP4的干扰效率及处理组PI3K/Akt信号通路相关基因的表达。结果显示RBP4的基因及蛋白的干扰效率达到60%(P<0.01)以上。进一步研究发现在胰岛素诱导及胰岛素抵抗的情况下,LH1-shRBP4干扰后可显著提高胰岛素信号通路AKT2、PI3K、GLUT4和IRS1基因mRNA的表达;明显促进AKT2、PI3K和IRS1蛋白的磷酸化;提高AKT2、PI3K和GLUT4基因的总蛋白水平。总之,RBP4干扰通过上调PI3K/Akt胰岛素信号通路相关因子的表达及其磷酸化水平,提高了胰岛素敏感性。此研究将为胰岛素抵抗相关疾病的治疗提供新思路。
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| 关 键 词： | 视黄醇结合蛋白4 慢病毒 前体脂肪细胞 猪 胰岛素信号通路 |
| 收稿时间： | 2012-10-08 |
Effects of retinol binding protein 4 knockdown on the PI3K/Akt pathways in porcine adipocytes |
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| Lei Pu,Jia Cheng,Guofang Wu,Hao Yang,Yang Qiu,Zhenyu Zhang,Gongshe Yang and Shiduo Sun. Effects of retinol binding protein 4 knockdown on the PI3K/Akt pathways in porcine adipocytes[J]. Chinese journal of biotechnology, 2013, 29(4): 447-457 |
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| Authors: | Lei Pu Jia Cheng Guofang Wu Hao Yang Yang Qiu Zhenyu Zhang Gongshe Yang Shiduo Sun |
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| Affiliation: | Laboratory of Animal Fat Deposition and Muscle Development, Northwest A & F University, Yangling 712100, Shaanxi, China;Laboratory of Animal Fat Deposition and Muscle Development, Northwest A & F University, Yangling 712100, Shaanxi, China;Laboratory of Animal Fat Deposition and Muscle Development, Northwest A & F University, Yangling 712100, Shaanxi, China;Laboratory of Animal Fat Deposition and Muscle Development, Northwest A & F University, Yangling 712100, Shaanxi, China;Laboratory of Animal Fat Deposition and Muscle Development, Northwest A & F University, Yangling 712100, Shaanxi, China;Laboratory of Animal Fat Deposition and Muscle Development, Northwest A & F University, Yangling 712100, Shaanxi, China;Laboratory of Animal Fat Deposition and Muscle Development, Northwest A & F University, Yangling 712100, Shaanxi, China;Laboratory of Animal Fat Deposition and Muscle Development, Northwest A & F University, Yangling 712100, Shaanxi, China |
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| Abstract: | |
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| Keywords: | retinol-binding protein 4 (RBP4) lentivirus vectors preadipocyte pig insulin signal pathway |
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