花莲种质资源的遗传多样性及品种间亲缘关系的探讨

利用17个随机引物对来自中国和美国的29份花莲种质资源材料进行了RAPD分析。扩增形成207条谱带，多态带122条，多态率为58．94％。说明中国花莲具有较丰富的遗传多样性。结果还显示：（1）花莲种质资源可被分为2个品种群：品种群Ⅰ以大花型为主，少量小花型。品种群Ⅰ以中小型花为主。又在欧氏遗传距离11．01处分为7个亚品种群。（2）美洲黄莲与中国莲的花莲之间在DNA水平上没有显著差异，与中小型花关系更近。

Genetic Diversity and Phylogenetic Relationship of Flower-lotus Cultivars (Nelumbo) by RAPD Markers

Random amplified polymorphic DNA(RAPD) was applied to germplasm accessions in 29 flower-lotus cultivars(including both Nelumbo nucifera Gaertn.and N.lutea Pers.).A total of 207 bands were obtained from 17 selected primers,of which 122 were polymorphic.The results also showed that:(1) Two main groups were gained:those cultivars,with bigger flower,were mainly clustered in one group,and the other contained mass small-flower types.(2) No obvious difference was observed between N.lutea and all other cultivars(N.nucifera) from their DNA.Furthermore,it seemed that N.lutea was close to small-flower cultivars of N.nucifera.