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Comparative analysis of cell surface proteins in chronic and acute leukemia cell lines |
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| Authors: | Lee Soo Jae Kim Kyun-Hwan Park Ji Sook Jung Jin Woo Kim Young Hwan Kim Sang Kyung Kim Wan-Seok Goh Hyun-gyung Kim Soo-hyun Yoo Jung-Sun Kim Dong-Wook Kim Kwang Pyo |
| Institution: | a Department of Molecular Biotechnology, Institute of Biomedical Science and Technology, Konkuk University, Seoul 143-701, Republic of Korea b Department of Pharmacology, School of Medicine, Konkuk University, Chungju, Republic of Korea c Proteome Analysis Team, Korea Basic Science Institute, Daejon 305-333, Republic of Korea d Microsystem Research Center, Korea Institute of Science and Technology, Seoul, Republic of Korea e Division of Hematology, St. Mary’s Hospital, The Catholic University of Korea, 62 Yeouido-dong, Yeongdeungpo-gu, Seoul 150-713, Republic of Korea |
| Abstract: | This study was designed to identify the cell surface protein markers that can differentiate between chronic myeloid leukemia (CML) and acute promyelocytic leukemia cells (APL). The differentially expressed plasma membrane proteins were analyzed between CML cell line (K562) and APL cell line (NB4) using the comparative proteomic approach. The cell membrane proteins were enriched by labeling with a membrane-impermeable biotinylation reagent, sulfo-NHS-SS-Biotin, and subjected to liquid chromatography tandem mass spectrometry (LC-MS/MS). By comparative proteomic analysis of K562 and NB4 cells, we identified 25 membrane and 14 membrane-associated proteins. The result of LC-MS/MS combined with chemical tagging method was validated by confirming the expression and localization of one of the differentially expressed plasma membrane proteins, CD43, by FACS and confocal microscopy. Our results indicate that CD43 could be a potential candidate for differentiating CML from APL. |
| Keywords: | Proteomics Chronic myeloid leukemia Acute promyelocytic leukemia Mass spectrometry |
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