野生红花白芨再生体系的建立和优化

以野生红花白芨成熟蒴果为外植体，建立了白芨植株的再生体系。结果表明：白芨种子萌发最佳培养基为1/2 MS+6-BA 1.0 mg·L^-1+NAA 0.1 mg·L^-1+AC 0.5 g·L^-1；原球茎增殖最适培养基为MS+KT 1.0 mg·L^-1+NAA 0.1 mg·L^-1+AC 0.5 g·L^-1；原球茎分化培养基为MS+6-BA 0.5 mg·L^-1+KT 1.0 mg·L^-1+NAA 0.1 mg·L^-1+AC 0.5 g·L^-1；不定芽增殖最佳培养基为MS+6-BA 0.5 mg·L^-1+KT 1.0 mg·L^-1+NAA 0.1 mg·L^-1+AC 0.5 g·L^-1 +土豆汁150 g·L^-1，30 d后的芽增殖率达343%；最适生根培养基为1/2MS+IBA 0.5 mg·L^-1+AC 0.5 g·L^-1+土豆汁100 g·L^-1，生根率达100%；试管苗移栽的最佳方式是以水苔+树皮+椰糠+锯木粉(2∶2∶1∶1，V/V/V/V)为混合基质进行多株移栽，成活率可达99%。

Establishment and Optimization of Wild Beltilla striata Regeneration System

We used the ripe capsules from wild Bletilla striata as explants to establish regeneration system. The optimal medium for seed germination was 1/2MS+6-BA 1.0 mg·L^-1+NAA 0.1 mg·L^-1+AC 0.5 g·L^-1. The most suitable medium for PLB multiplication was MS+KT 1.0 mg·L^-1+NAA 0.1 mg·L^-1+AC 0.5 g·L^-1, while MS+6-BA 0.5 mg·L^-1+KT 1.0 mg·L^-1+NAA 0.1 mg·L^-1+AC 0.5 g·L^-1 was appropriate for PLB differentiation. The optimal medium for adventitious buds multiplication was MS+6-BA 0.5 mg·L^-1+KT 1.0 mg·L^-1+NAA 0.1 mg·L^-1+AC 0.5 g·L^-1+potato juice 150 g·L^-1, and the proliferation rate was up to 343% after 30 days. The most suitable rooting medium was 1/2MS+IBA 0.5 mg·L^-1+AC 0.5 g·L^-1+potato juice 100 g·L^-1 with the rooting rate of 100%. The best way of tube seedlings transplanting was under the volume ratio of sphagna: batk: Coconut chaff: wood flour of 2∶2∶1∶1 with 99% of survival rate.