葡萄风信子叶片及花器官组织培养研究

以4种葡萄风信子Muscari armeniacum、Muscari aucheri ‘Dark Eyes’、Muscari aucheri ‘Ocean Magic’、Muscari azureum的叶片以及M.aucheri ‘Dark Eyes’的花器官为外植体，进行诱导愈伤组织分化的研究，建立了葡萄风信子的组织培养体系。研究结果表明：葡萄风信子抽梗期叶片最适升汞灭菌时间为7 min；筛选出四种品种的叶片最佳诱导愈伤组织培养基均为：MS+2，4-D 0.5 mg·L^-1+TDZ 0.1 mg·L^-1；同一叶片不同部位的愈伤组织诱导率由高到低依次是叶片基部、叶片中部、叶片尖端；M.aucheri ‘Dark Eyes’品种的花器官出愈率由高到低依次是花梗、花瓣、花药；筛选出M.aucheri ‘Dark Eyes’花药的最佳诱导培养基：MS+2,4-D 0.1 mg·L^-1+6-BA 0.5 mg·L^-1。

Tissue Culture System of Muscari botryoides Mill Leaf and Flower Organs

We studied the tissue culture of Muscari botryoides Mill from four species of leaves and flower organs of Muscari aucheri ‘Dark Eyes’ with different concentrations of hormones, and established an efficient tissue culture system. With the variety of Muscari armeniacum,the most suitable sterilization time of mercuric chloride was 7 min. Callus could be induced from the leaves of every variety, and the appropriate medium was MS+2,4-D 0.5 mg·L^-1+TDZ 0.1 mg·L^-1. Different parts of one leaf showed high to low induction rate in the order of leaf base, leaf middle and leaf tip. Flower organs of M.aucheri ‘Dark Eyes’ showed high to low induction rate in the order of pedicels, petal and anthers. The optimal induction medium of anther was MS+2,4-D 0.1 mg·L^-1+6-BA 0.5 mg·L^-1.