Imaging of immobilized enzyme spots by scanning chemiluminescence microscopy with electrophoretic injection |
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Authors: | Hirano Yu Mitsumori Yutaro Oyamatsu Daisuke Nishizawa Matsuhiko Matsue Tomokazu |
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Affiliation: | Department of Biomolecular Engineering, Graduate School of Engineering, Tohoku University, 07 Aramaki-Aoba, Aoba-ku, 980-8579, Sendai, Japan. |
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Abstract: | Scanning chemiluminescence microscopy (SCLM) with electrophoretic injection was developed and applied to visualize enzyme reactions localized in an enzyme microspot. The SCLM uses a tapered glass capillary as a probe for injecting a small amount of luminol onto the substrate to generate localized chemiluminescence. The electrophoretic injection by application of a constant current between the inside and outside of the capillary enabled the continuous and controllable injection of a minute quantity of luminol in the range of 0.1 pmol/s. The image of enzyme activity in a monolayer spot of horseradish peroxidase was obtained by using the electrophoretic injection-based SCLM system. |
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