Localization to chromosomes of structural genes for the major protease inhibitors of barley grains |
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Authors: | J. Hejgaard S. E. Bjørn G. Nielsen |
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Affiliation: | (1) Department of Biochemistry and Nutrition, Technical University of Denmark, DK-2800 Lyngby, Denmark;(2) Agricultural Research Department, Risø National Laboratory, DK-4000 Roskilde, Denmark |
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Abstract: | Summary Wheat-barley chromosome addition lines were compared by isoelectric focusing of protein extracts to identify chromosomes carrying loci for the major immunochemically distinct protease inhibitors of barley grains. Structural genes for the following inhibitors were localized: an inhibitor of both endogenous -amylase 2 and subtilisin (ASI) on chromosome 2, two chymotrypsin/subtilisin inhibitors (CI-1 and CI-2) on chromosome 5 (long arm) and the major trypsin inhibitor (TI-1) on chromosome 3. |
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Keywords: | Addition line Barley Isoelectric focusing Protease inhibitors Structural gene |
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