Molecular cloning and cDNA structure of the regulatory subunit of type I cAMP-dependent protein kinase from rat brain |
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Authors: | T Kuno Y Ono M Hirai S Hashimoto H Shuntoh C Tanaka |
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Abstract: | Complementary DNA (cDNA) clones encoding the regulatory subunit of the type I cAMP-dependent protein kinase (R-I) were isolated by screening of rat brain cDNA libraries. A 1.5-kilobase (kb) cDNA insert containing the entire coding region was sequenced and full amino acid sequence has been deduced from the nucleotide sequence. The clone encodes for a protein of 380 amino acids that shows 97% homology to the bovine R-I subunit. Northern blot analysis demonstrated two major mRNA species (2.8 and 4.4 kb in size) in rat brain and liver. |
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