Synthesis and evaluation of polymeric continuous bed (monolithic) reversed-phase gradient stationary phases for capillary liquid chromatography and capillary electrochromatography |
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| Institution: | 1. Alkali Soil Natural Environmental Science Center, Northeast Forestry University, Key Laboratory of Saline-alkali Vegetation Ecology Restoration in Oil Field, Ministry of Education, Harbin 150040, China;2. Department of Biology, Genetics Institute, Plant Molecular and Cellular Biology Program, Interdisciplinary Center for Biotechnology Research, University of Florida, Gainesville, FL 32610, USA;1. Institute of Electronic Materials Technology, Wolczynska 133, 01-919 Warsaw, Poland;2. Faculty of Chemistry, University of Warsaw, Pasteura 1, 02-093 Warsaw, Poland;3. Faculty of Physics, University of Warsaw, Hoza 69, 00-681 Warsaw, Poland;4. Industrial Chemistry Research Institute, Rydygiera 8, 01-793 Warsaw, Poland;5. Faculty of Materials Engineering, Warsaw University of Technology, Woloska 141, 02-507 Warsaw, Poland |
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| Abstract: | There is a demand of novel high resolution separation media for separation of complex mixtures, particularly biological samples. One of the most flexible techniques for development of new separation media currently is synthesis of the continuous bed (monolithic) stationary phases. In this study the capillary format gradient stationary phases were formed using continuous bed (monolith) polymerization in situ. Different reversed-phase stationary phase gradients were tailored and their resolution using capillary liquid chromatography and capillary electrochromatography at isocratic mobile phase conditions was evaluated. It is demonstrated, that efficiency and resolution of the gradient stationary phases can be substantially increased comparing to the common (isotropic) stationary phases. The proposed formation approach of the gradient stationary phase is reproducible and compatible with the capillary format or microchip format separations. It can be easily automated for the separation optimizations or mass production of the capillary columns or chips. |
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