A simple and accurate method for quantification of magnetosomes in magnetotactic bacteria by common spectrophotometer |
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| Affiliation: | 1. Department of Microbiology, Ludwig Maximilian University of Munich, Germany;2. Department of Microbiology, Ernst Moritz Arndt University of Greifswald, Germany;3. Department of Life Sciences, The National Institute for Biotechnology in the Negev, Ilse Katz Institute for Nanoscale Science and Technology, Ben-Gurion University of the Negev, Beersheba, Israel;4. Department of Microbiology, University of Bayreuth, Germany;1. Dpto. de Electricidad y Electrónica, Universidad del País Vasco - UPV/EHU, Leioa 48940, Spain;2. Dpto. de Física Aplicada I, Universidad del País Vasco - UPV/EHU, Bilbao 48013, Spain;3. BCMaterials, Parque tecnológico de Zamudio, Derio 48160, Spain;4. Dpto. de Inmunología, Microbiología y Parasitología, Universidad del País Vasco - UPV/EHU, Leioa 48940, Spain;5. CITIMAC, Universidad de Cantabria, Santander 39005, Spain;6. SGIker, Universidad del País Vasco - UPV/EHU, Leioa 48940, Spain |
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| Abstract: | A simple apparatus for measuring the magnetism of magnetotactic bacteria was developed with a common laboratory spectrophotometer, which was based on measuring the change in light scattering resulting from cell alignment in a magnetic field. A multiple coils were built around the cuvette holder of the spectrophotometer to compensate geomagnetic field and to generate two mutually perpendicular magnetic fields. In addition, we defined a novel magnetism parameter, Rmag, by modifying the definition of Cmag to a normalized parameter with the culture absorbance obtained without application of magnetic field. The number of magnetosomes in each cell was determined by transmission electron microscopy to assess the relationship between the two magnetism parameters and the distribution of magnetosomes in the cells. We found that both Rmag and Cmag were linearly correlated rather with the percentage of magnetosome-containing bacteria than with the average magnetosome numbers, and Rmag exhibited a better linearity than Cmag with respect to the percentage of magnetosome-containing bacteria. |
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