Ultrasound-assisted digestion: A useful alternative in sample preparation |
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Affiliation: | 1. Department of Chemistry, University of Siena, Via A. Moro 2, 53100 Siena, Italy;2. Faculty of Chemistry, University of Wroclaw, F. Joliot-Curie 14, 50383 Wroclaw, Poland;1. Instituto Nacional de Tecnología Industrial, Centro de Investigación y Desarrollo en Mecánica, Av. General Paz 5445 B1650KNA, San Martín, Buenos Aires, Argentina;2. Instituto de Investigación e Ingeniería Ambiental (3iA), Universidad de San Martín, Campus Miguelete, Martín de Irigoyen 3100, 1650, San Martín, Buenos Aires, Argentina;3. Comisión Nacional de Energía Atómica, Gerencia Química, Av. Gral Paz 1499, B1650KNA, San Martín, Buenos Aires, Argentina;4. Comisión Nacional de Energía Atómica, Gerencia Investigación y Aplicaciones, Av. Gral Paz 1499, B1650KNA, San Martín, Buenos Aires, Argentina;5. Consejo Nacional de Investigaciones Científicas y Técnicas, Av. Rivadavia 1917, C1033AAJ, Buenos Aires, Argentina |
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Abstract: | Ultrasound-assisted digestion is a promising alternative in the analysis of solid samples when either simple dissolution or direct analysis is not applicable. However, the field of application of ultrasonic sample digestion is still small in comparison with classical digestion alternatives and, particularly, with microwave-assisted digestion. This fact can be justified by the scant knowledge analytical chemists have about the advantages ultrasonic energy provides to digestion. Among these, the strict control at low temperatures of ultrasound applications allows the implementation of ultrasonic-assisted steps in biochemical analyses. In this connection, two specific biological applications, ultrasonic enzymatic digestion and assistance of ultrasound for cell disruption, are also reviewed. |
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