Enantioseparation by ligand-exchange using particle-loaded monoliths: Capillary-LC versus capillary electrochromatography |
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| Affiliation: | 1. Department of Chemistry, University of Calcutta, 92 APC Road, Kolkata 700009, India;2. Department of Chemistry, Dr. B. N. Dutta Smriti Mahavidyalaya, Burdwan 713407, India;3. Division of Science and Engineering, Penn State University Abington, Abington, PA 19001, USA;4. Department of Chemistry, CSIR – Indian Institute of Chemical Biology, 4, Raja SC Mullick Road, Kolkata 700032, India;1. State Key Laboratory of Food Additive and Condiment Testing, Zhenjiang Entry-exit Inspection Quarantine Bureau, Zhenjiang 212008, China;2. Research Institute of Lanzhou Petrochemical Corporation, Lanzhou 730060, China;3. Animal, Plant and Food Inspection Center, Jiangsu Entry-exit Inspection Quarantine Bureau, Nanjing 210001, China;4. Key Laboratory of Energy and Chemical Engineering, Ningxia University, Yinchuan 750021, China;1. Department of Chemistry and Biochemistry, University of Texas at Arlington, 700 Planetarium Place, Arlington, TX 76019, United States;2. Department of Chemistry, Kyungpook National University, Daegu, 702-701, Republic of Korea;1. Institute of Pharmaceutical Sciences, Pharmaceutical (Bio-)Analysis, University of Tübingen, Auf der Morgenstelle 8, 72076, Tübingen, Germany;2. Department of Pharmaceutical Sciences, University of Perugia, Via Del Liceo 1, 06123, Perugia, Italy |
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| Abstract: | Particle-loaded monoliths containing a polymethacrylamide backbone were prepared by suspending a silica-based chiral phase in the mixture of the monomers followed by in-situ polymerization in the capillary. As chiral selector l-4-hydroxyproline chemically bonded to 3 μm silica particles was used following the separation principle of ligand-exchange. Electrolytes containing Cu(II) ions were used. Amino acid enantiomers were separated by capillary-LC and CEC, whereby the latter showed the better resolution properties. For the chiral separation of α-hydroxy acids the EOF was reversed by copolymerizing diallyldimethylammonium chloride instead of vinylsulfonic acid as charge providing agent. Short columns of 6 cm were found to be sufficient in the case of CEC for baseline separations of amino acids with α values up to 5. |
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