Role of carbon nano-materials in the analysis of biological materials by laser desorption/ionization-mass spectrometry |
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| Institution: | 1. CAS Key Laboratory of Separation Science for Analytical Chemistry, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian, Liaoning, 116023, People’s Republic of China;2. University of Chinese Academy of Sciences, Beijing, 100049, People’s Republic of China;3. Department of Anesthesiology, The First Affiliated Hospital of Harbin Medical University, Harbin, Heilongjiang, 150001, People’s Republic of China;1. CAS Key Laboratory of Separation Science for Analytical Chemistry, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian, 116023, People’s Republic of China;2. University of Chinese Academy of Sciences, Beijing, 100039, People’s Republic of China;1. Department of Materials Science and Engineering, Yonsei University, 134 Shinchon-dong, Seodaemun-gu, Seoul 120-749, Republic of Korea;2. Department of Chemistry, Ewha Womans University, Seoul, Republic of Korea;3. Korea Institute of Science and Technology (KIST), Seoul, Republic of Korea;1. Key Laboratory of Microbial Technology for Industrial Pollution Control of Zhejiang Province, College of Environment, Zhejiang University of Technology, Hangzhou 310014, People''s Republic of China;2. Key Laboratory of Tea Biology and Resources Utilization, Ministry of Agriculture, Tea Research Institute, Chinese Academy of Agricultural Sciences, Hangzhou 310008, People''s Republic of China;3. Key Laboratory of Separation Science for Analytical Chemistry, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian 116023, People''s Republic of China;1. Department of Chemistry, National Sun Yat-Sen University, Kaohsiung 804, Taiwan;2. Department of Chemistry, Assuit University, Assuit 71515, Egypt;3. College of Pharmacy, Kaohsiung Medical University, Kaohsiung 807, Taiwan;4. Center for Nanoscience and Nanotechnology, National Sun Yat-Sen University, Kaohsiung 804, Taiwan;5. Institute of Medical Science and Technology, National Sun Yat-Sen University, 80424 Taiwan;6. Doctoral Degree Program in Marine Biotechnology, National Sun Yat-Sen University, Kaohsiung 804, Taiwan;1. Institute of Microanalytical Systems, Department of Chemistry, Zhejiang University, Hangzhou 310058, China;2. Institute of Cancer Research, School of Medicine, Zhejiang University, Hangzhou 310009, China;3. Hospital of Zhejiang University, Hangzhou 310058, China |
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| Abstract: | At present, carbon nano-materials are being utilized in various procedures, especially in laser desorption/ionization-mass spectrometry (LDI-MS) for analyzing a range of analytes, which include peptides, proteins, metabolites, and polymers. Matrix-oriented LDI-MS techniques are very well established, with weak organic acids as energy-absorbing substances. Carbon materials, such as nano-tubes and fullerenes are being successfully applied in the small-mass range, where routine matrices have strong background signals. In addition, the role of carbon nano-materials is very well established in the fractionation and purification fields. Modified diamond powder and surfaces are utilized in binding peptides and proteins from complex biological fluids and analyzed by matrix-assisted laser desorption/ionization (MALDI) time-of-flight (TOF) mass spectrometry (MS). Polylysine-coated diamond is used for solid-phase extraction to pre-concentrate DNA oligonucleotides. Graphite is useful for desalting, pre-concentration, and as energy-absorbing material (matrix) in desorption/ionization. Carbon nano-tubes in their different derivatized forms are used as matrix materials for the analysis of a range of analytes, such as carbohydrates, amino acids, peptides, proteins, and some environmental samples by LDI-MS. Fullerenes are modified in different ways to bind serum entities analyzed through MALDI/TOF-MS and are subsequently utilized in their identifications. In addition, the fullerenes are a promising matrix in LDI-MS, but improvements are needed. |
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