Proteomic analysis of mouse growth plate cartilage |
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| Authors: | Belluoccio Daniele Wilson Richard Thornton David J Wallis Tristan P Gorman Jeffrey J Bateman John F |
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| Institution: | Murdoch Childrens Research Institute and the Department of Paediatrics, University of Melbourne, Royal Children's Hospital, Parkville, Victoria, Australia. |
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| Abstract: | Cartilage is a highly specialized load-bearing tissue with a small number of cells and a high proportion of extracellular matrix (ECM). The abundance of heavily sulfated proteoglycans and a poorly soluble collagenous ECM presents a major technical challenge to 2-DE. Here we report proteomic analysis of mouse growth plate cartilage using novel methodology for tissue dissection and sample prefractionation. We have successfully resolved cartilage tissue extracts by 2-DE for the first time and identified cartilage ECM proteins by Western blotting and MS/MS. |
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| Keywords: | Cartilage Extracellular matrix proteins IEF optimization Prefractionation |
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