Enzymatic synthesis of ATP from RNA and adenine |
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Authors: | V N Barai A I Zinchenko L M Zalashko L A Eroshevskaya I A Mikhailopulo |
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Institution: | (1) Institute of Microbiology Academy of Sciences of Belarus, 220141 Minsk, Republic of Belarus;(2) Institute of Bioorganic Chemistry, Academy of Sciences of Belarus, 220141 Minsk, Republic of Belarus |
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Abstract: | Summary The title compound was prepared by a three-stage enzymatic procedure consisting of (i) RNA hydrolysis to a mixture of ribonucleosides using intact mycelium of Spicaria violacea, (ii) transribosylation of exogenous adenine employing whole cells of Escherichia coli as a biocatalyst, and (iii) conversion of formed adenosine into ATP by the enzymes of alcohol fermentation and the kinases extracted from baker's yeast. |
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