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Enzymatic synthesis of ATP from RNA and adenine
Authors:V N Barai  A I Zinchenko  L M Zalashko  L A Eroshevskaya  I A Mikhailopulo
Institution:(1) Institute of Microbiology Academy of Sciences of Belarus, 220141 Minsk, Republic of Belarus;(2) Institute of Bioorganic Chemistry, Academy of Sciences of Belarus, 220141 Minsk, Republic of Belarus
Abstract:Summary The title compound was prepared by a three-stage enzymatic procedure consisting of (i) RNA hydrolysis to a mixture of ribonucleosides using intact mycelium of Spicaria violacea, (ii) transribosylation of exogenous adenine employing whole cells of Escherichia coli as a biocatalyst, and (iii) conversion of formed adenosine into ATP by the enzymes of alcohol fermentation and the kinases extracted from baker's yeast.
Keywords:
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