利用PLFA、CLPPs和ARDRA标记分析甲胺磷对土壤微生物群落的影响

利用磷脂脂肪酸(PLFA)、群落水平生理活性(CLPPs)和扩增核糖体DNA限制性分析(ARDRA)标记,综合评估低浓度和高浓度甲胺磷连续施用2和4 yr后对土壤微生物群落结构、功能和遗传多样性的影响。结果表明,甲胺磷胁迫使土壤微生物生物量减少而细菌(革兰氏阴性菌)数量增加,同时使微生物群落功能多样性下降而遗传多样性提高;处理2 yr后高浓度甲胺磷胁迫对微生物群落的影响较低浓度胁迫更为明显,处理4 yr后两个浓度胁迫则具有相似的显著影响,表明不同浓度甲胺磷的长期胁迫均能对微生物群落造成严重破坏。

Effects of methamidophos on soil microbial communities measured by PLFA, CLPPs and ARDRA markers

With a combined approach of phospholipid fatty acid(PLFA),community level physiological profiles(CLPPs) and amplified ribosomal DNA restriction analysis(ARDRA),this paper evaluated the effects of methamidophos continuously applied for 2 and 4 years on the structural,functional and genetic diversities of soil microbial communities.The results showed that the application of methamidophos reduced the microbial biomass but enhanced the quantity of Gram-negative bacteria,and increased the functional diversity of micriboal communities while decreased their genetic diversity.After 2 years application,high dosage methamidophos had more significant effect on the microbial communities than low dosage methamidophos,while after 4 years application,these two dosages had the similar significant effect,suggesting that different concentrations of methamidophos applied for a long period all had a distinguishable and severe impact on soil microbial communities.