Cell division and death in the mouse blastocyst before implantation |
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Authors: | Alan H Handyside Susan Hunter |
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Institution: | (1) MRC Experimental Embryology and Teratology Unit, Medical Research Council Laboratories, Woodmansterne Road, SM5 4EF Carshalton, Surrey, Great Britain |
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Abstract: | Summary The numbers of cells in the trophectoderm (TE) and inner cell mass (ICM) of mouse blastocysts were counted by differentially labelling their nuclei with two polynucleotide-specific fluorochromes. Blastocysts recovered from the uterus at intervals between their formation early on Day 4 to the initial stages of implantation on day 5 were analysed. TE cell number increase was initially rapid, indicating some synchronisation of the sixth division, but slowed down progressively and plateaued on Day 5, possibly due to the onset of primary giant cell formation. ICM cell number increase was slower than the corresponding TE cells. As a result, TE cell number more than quadrupled, whereas ICM cell number only doubled over this period. Although the mitotic index of both populations of cells fell steadily, there was no significant difference between them. The decline in the proportion of ICM cells, therefore, is likely to be due to cell death, first detected in early blastocysts and predominantly located in the ICM. In addition, however, a contribution of ICM cells to the overlying polar TE cannot be excluded. |
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Keywords: | Mouse blastocyst Trophectoderm Inner cell mass Cleavage Cell death |
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