烟青虫质型多角体病毒的形态及某些理化特性

本文对烟青虫(Heliothis assulta)质型多角体病毒(CPV)的形态大小以及某些理化特性进行了研究。烟青虫CPV多角体为正五角形的十二面体，大小为0.8-4.6μm；CPV 粒子为外形呈六角形或球形的廿面体，大小为62nm。CPV多角体蛋白的主要多肽为一种，分子量23000，为非糖蛋白。 用SDS-酚法提取的CPV基因，经Rnase I和Dnase I处理后在1%琼脂糖凝胶上电泳，结果表明 其基因是双链RNA,并由10个基因片段组成。各片段大小为0.3-2.68X10⁶,总分子量为15.85x10⁶。本文所报道的烟青虫质型多角体病毒在国内外尚属首次。

MORPHOLOGY AND BIOCHEMICAL CHARACTERISTICS OFA CYTOPLASMIC POLYHEDROSIS VIRUS FROMTOBACO BUDWORM HELIOTHIS ASSULTA

A cytoplasmic polyhedrosis virus (CPV) isolated recently from the tobacco budworm Heliothis assulta was observed with EM and characterized biochemically. The polyhedral bodies of the CPV were pentagonal dodecahedron measured 0.8 to 4.6μm, and the virioas after release from the polyhedra were found with a hexagonal or spherical outline and a size of 62nm. The polyhedrin had a molecular weight of23,000 dalton and was not a glycoprotein because it was stained negatively by periodic acid-Schiff's reagent. Genes of the CPV were double-stranded with Dnase I resistance and considerable Rnase I resistance. The CPV genome consisted of ten segments of RNA. Their molecular weights range from 0.3-2.68 ×10⁶ dalton, with a total of 15.85×10⁶ dalton.