Loss of antibody productivity is highly reproducible in multiple hybridoma subclones |
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Authors: | Merritt S E Palsson B O |
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Institution: | Cellular Biotechnology Laboratory, Department of Chemical Engineering, The University of Michigan, Ann Arbor, Michigan 48109. |
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Abstract: | An immunoglobulin G (IgG(2b)) producing hybridoma cell line (S3H5/gamma2bA2) was cloned and subcloned. Twenty subclones were grown in parallel while being adapted in a stepwise fashion to serum-free medium. Following adaptation to serum-free medium, it was found that 16 of the 20 subclones remained at a relatively constant proportion of nonproducing cells. Three of the remaining subclones transiently deviated from this balance but eventually returned toward this population composition. One subclone continued to lose productivity. A population balance was reached at approximately 8% of the population being nonproducers. The loss of antibody productivity was thus highly reproducible. (c) 1993 John Wiley & Sons, Inc. |
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Keywords: | hybridoma antibody productivity kinetics instability |
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