Human alpha-chain globin messenger: prediction of a nucleotide sequence |
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| Authors: | B Laux D Dennis H B White |
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| Affiliation: | 1. Veterans Administration Hospital, Memphis, Tennessee 38104 USA;2. the Department of Biochemistry, University of Tennessee Medical Units, Memphis, Tennessee 38104 USA |
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| Abstract: | The presence in human serum of inhibitory activity to rat liver insulin specific protease has been detected in an alpha1 globulin preparation (Cohn Fraction IV1). Separation into four components and partial purification (40 to 107 fold) has been achieved by heat denaturation of non-active protein, Sephadex G-100 gel filtration and ion-exchange chromatography upon QAE Sephadex. Each of the inhibitors was found to be competitive in nature. The molecular weight of the inhibitors is between 4,000–7,000 and the activity is destroyed for the most part by chymotrypsin. |
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