The inhibitory effect of serum on cell attachment can be prevented by cobalt-protoporphyrin IX |
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Authors: | C Verger J Imbenotte |
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Affiliation: | ER 303 C.N.R.S., I.R.S.C., Villejuif, France. |
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Abstract: | The rate of attachment of chick embryo fibroblasts (CEF) incubated for 1 h at pH 6 in the absence of serum, was equal to about 90% with 2% of spreading cells. The addition of 1% horse serum (HS) decreased to about 30% the rate of attached cells but increased to about 30% the proportion of spreading cells. We found that 3 microM cobalt-protoporphyrin IX (CoPP) added for 3 min before the addition of 1% HS increased both the rate of cell attachment (about 60%) and the proportion of cell spreading (about 50%). |
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