Hydrolysis of N'-benzoyl-d-arginine-p-nitroanilide by members of the Haloanaerobiaceae: additional evidence that Haloanaerobium praevalens is related to endospore-forming bacteria |
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Authors: | Aharon Oren Lia V. Gofshtein-Gandman Alexander Keynan |
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Affiliation: | Division of Microbial and Molecular Ecology, Institute of Life Sciences, Hebrew University of Jerusalem, Jerusalem, Israel;Department of Biological Chemistry, Institute of Life Sciences, Hebrew University of Jerusalem, Jerusalem, Israel |
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Abstract: | Abstract Three out of the four described halophilic obligately anaerobic bacteria of the family Haloanaerobiaceae hydrolyze d -BAPA (N'-benzoyl- d -arginine-p-nitroanilide), while showing no or little l -BAPA hydrolyzing activity. This property was shown earlier to be characteristic only of non-halophilic Gram-positive endospore-forming bacteria of the genera Bacillus and Clostridium . These results suggest that Haloanaerobium praevalens , which has never been shown to produce endospores, but was shown to be related to the endosphere-forming representatives of the Haloanaerobiaceae on the basis of 16S rRNA nucleotide sequence data, shares other properties characteristics of the endospore-forming bacteria. Neither significant d -BAPA nor l -BAPA hydrolyzing activity was found in Sporohalobacter lortetii . |
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Keywords: | d-BAPA hydrolysis Haloanaerobiaceae Haloanaerobium praevalens Endospores |
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