Development and characterisation of microsatellite loci in Farfugium japonicum (Asteraceae) |
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| Authors: | Naofumi Nomura Kento Fujiwara Tokushiro Takaso Motomi Ito Koichi Uehara Hiroaki Setoguchi |
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| Institution: | (1) Research Institute for Humanity and Nature, Kamigamo Motoyama, Kita, Kyoto 603-8047, Japan;(2) Graduate School of Arts and Sciences, Tokyo University, Meguro, Komaba, Tokyo 153-8902, Japan;(3) Graduate School of Horticulture, Chiba University, Matsudo, Matsudo 271-8510, Japan;(4) Graduate School of Human and Environmental Studies, Kyoto University, Yoshida Nihonmatsu, Sakyo, Kyoto 606-8501, Japan |
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| Abstract: | Eight microsatellite loci for the perennial herb Farfugium japonicum, including the rheophytic variety luchuense endemic to riparian areas of the Ryukyu Islands, Japan, were isolated and characterised. The number of alleles ranged from
5 to 14. The expected (H
E) and observed (H
O) heterozygosities were 0.344–0.885 and 0.121–0.754, respectively, from 69 individuals in one population. Six loci exhibited
significantly fewer heterozygotes than expected under Hardy–Weinberg equilibrium (P < 0.05). The primers amplifying microsatellite sequences in F. japonicum may provide a population genetics tool useful in the establishment of a conservation strategy. |
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| Keywords: | Conservation Asteraceae Heterozygosity Microsatellite Farfugium |
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