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Development and characterisation of microsatellite loci in Farfugium japonicum (Asteraceae)
Authors:Naofumi Nomura  Kento Fujiwara  Tokushiro Takaso  Motomi Ito  Koichi Uehara  Hiroaki Setoguchi
Institution:(1) Research Institute for Humanity and Nature, Kamigamo Motoyama, Kita, Kyoto 603-8047, Japan;(2) Graduate School of Arts and Sciences, Tokyo University, Meguro, Komaba, Tokyo 153-8902, Japan;(3) Graduate School of Horticulture, Chiba University, Matsudo, Matsudo 271-8510, Japan;(4) Graduate School of Human and Environmental Studies, Kyoto University, Yoshida Nihonmatsu, Sakyo, Kyoto 606-8501, Japan
Abstract:Eight microsatellite loci for the perennial herb Farfugium japonicum, including the rheophytic variety luchuense endemic to riparian areas of the Ryukyu Islands, Japan, were isolated and characterised. The number of alleles ranged from 5 to 14. The expected (H E) and observed (H O) heterozygosities were 0.344–0.885 and 0.121–0.754, respectively, from 69 individuals in one population. Six loci exhibited significantly fewer heterozygotes than expected under Hardy–Weinberg equilibrium (< 0.05). The primers amplifying microsatellite sequences in F. japonicum may provide a population genetics tool useful in the establishment of a conservation strategy.
Keywords:Conservation  Asteraceae  Heterozygosity  Microsatellite            Farfugium
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