Biosynthesis of plant-specific phenylpropanoids by construction of an artificial biosynthetic pathway in <Emphasis Type="Italic">Escherichia coli</Emphasis> |
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Authors: | Oksik Choi Cheng-Zhu Wu Sun Young Kang Jong Seog Ahn Tai-Boong Uhm Young-Soo Hong |
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Institution: | (1) Korea Research Institute of Bioscience and Biotechnology, 685-1 Yangcheong-ri Ochang-eup, Chungbuk, 363-883, Republic of Korea;(2) Biological Sciences, Chunbuk National University, Jeollabuk-do, 561-756, Republic of Korea; |
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Abstract: | Biological synthesis of plant secondary metabolites has attracted increasing attention due to their proven or assumed beneficial
properties and health-promoting effects. Phenylpropanoids are the precursors to a range of important plant metabolites such
as the secondary metabolites belonging to the flavonoid/stilbenoid class of compounds. In this study, engineered Escherichia coli containing artificial phenylpropanoid biosynthetic pathways utilizing tyrosine as the initial precursor were established
for production of plant-specific metabolites such as ferulic acid, naringenin, and resveratrol. The construction of the artificial
pathway utilized tyrosine ammonia lyase and 4-coumarate 3-hydroxylase from Saccharothrix espanaensis, cinnamate/4-coumarate:coenzyme A ligase from Streptomyces coelicolor, caffeic acid O-methyltransferase and chalcone synthase from Arabidopsis thaliana, and stilbene synthase from Arachis hypogaea. |
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