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Application of the quasi-spectral density function of 15N nuclei to the selection of a motional model for model-free analysis |
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| Authors: | Rieko Ishima Kazuhiko Yamasaki Kuniaki Nagayama |
| Affiliation: | (1) Nagayama Protein Array Project, ERATO, JRDC, Tsukuba Research Consortium, Pilot Laboratory, 5-9-1 Tokodai, 300-26 Tsukuba, Japan;(2) Protein Engineering Research Institute, 6-2-3 Furuedai, 565 Suita, Osaka, Japan;(3) Department of Pure and Applied Sciences, College of Arts and Sciences, University of Tokyo, Komaba, 153 Meguro, Tokyo, Japan;(4) Division of Molecular and Structural Biology, Ontario Cancer Institute, Princess Margaret Hospital, 610 University Avenue, M5G 2M9 Toronto, ON, Canada |
| Abstract: | Summary Parameters used in model-free analysis were related to simulated spectral density functions in a frequency region experimentally obtained by quasi-spectral density function analysis of 15N nuclei. Five kinds of motional models used in recent model-free analyses were characterized by a simple classification of the experimental spectral density function. We demonstrate advantages and limitations of each of the motional models. To verify the character of the models, model selection using experimental spectral density functions was examined. |
| Keywords: | Protein Relaxation RNase HI |
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