Effects of thawing,refreezing and storage conditions of tissue samples and protein extracts on 2‐DE spot intensity |
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| Authors: | Stephanie Weist Cindy Brunkau Janosch Wittke Murat Eravci Oliver Broedel Eberhard Krause Heike Stephanowitz Selda Eravci Andreas Baumgartner |
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| Institution: | 1. Department of Radiology and Nuclear Medicine (Radiochemistry), Charité Universit?tsmedizin, Campus Benjamin Franklin, Berlin, Germany;2. A+M Proteome Science, Berlin, Germany;3. Leibniz Institute for Molecular Pharmacology (FMP), Berlin, Germany |
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| Abstract: | We report that reliable quantitative proteome analyses can be performed with tissue samples stored at ?80°C for up to 10 years. However, storing protein extracts at 4°C for 24 h and freezing protein extracts at ?80°C and thawing them significantly altered 41.6 and 17.5% of all spot intensities on 2‐DE gels, respectively. Fortunately, these storing effects did not impair the reliability of quantifying 2‐DE experiments. Nonetheless, the results show that freezing and storage conditions should be carefully controlled in proteomic experiments. |
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| Keywords: | 2‐DE Freezing Rat brain Storage Technology Thawing |
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